User:Tara K. Luckau/Notebook/Team ConGen/2012/03/30

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SCOC Multiplex Optimization

Fragment Analysis Results

SCOC MPY Uniplexes





for evenness

this is the best sample; most had extremely high Scun15 and extremely low Scun19, low Scun9

+ sar80

sar80 did not amplify at all when multiplexed with the green lane

SCOC Conclusions

  • Scun11 range is very large and overlaps with sar80
  • sar80 seems non polymorphic?
  • because of sar80's resistance to amplify and overlapping amplicon range with Scun11, needs to be run separately from the other 9 markers for SCOCs
  • may be able to integrate it with the 8 SW markers not yet screened

CNHY Multiplex Optimization

Fragment Analysis Results


throw it all together to see what happens

this is by far the best sample: many markers seemed to amplify (Ai5033, Ai5037, Acos5, Ai5035, Ai5062), only Ai5057's amplicon is not seen
all other samples only amplified Acos5

CNHY Conclusions

  • oye, lots of work still to do, here

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