User:Tara K. Luckau/Notebook/Team ConGen/2012/07/05

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  • decreasing gradient of Scun15 concentration (peak too high) (Scun9 and Scun19 concentrations held constant)

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 140 V
27 µL GelRed 6 µL ladder 50 minutes

  • three distinct bands visible for most conditions; bands occur in expected size ranges
  • differences between the two control DNAs are evident, and not expected - hopefully results will look consistent in fragment analysis regardless of source DNA
  • from gel view, predict 0.075µL/reaction Scun15 will be preferred
  • OVERALL: gel looks good - send to frag

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