User:Tara K. Luckau/Notebook/Team ConGen/2012/07/17

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Contents


SCOC MP1

PCR MP1-G+Y

  • try to combine MP1-G with MP1-Y (same buffer and annealing temperature, limited primer interactions)


CNHY/TI MP1

PCR Uniplex Ai5033

  • Uniplex


PCR MP1a and MP1b

  • Primer pairs grouped into the two multiplexes based on:
- primer interactions (especially interactions of concern)
- annealing temperature
- amplicon size
  • all primers at same concentration
  • GOAL: to see whether all loci are amplifying and at what intensity





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