User:Tim Henry/Notebook/D1S80/2012/02/20

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D1S80 w/ LB buffer

D1S80 gel run on 2012/02/20

Chelex Buccal extraction 2012/02/18

PCR on 2012/02/19


First time using LB buffer.

1.1% agarose. 75mL total. (.825g agarose, + 75mL LB)

280v for approx 45minutes. LB doc suggested 4x normal voltage.

Results look very smudged and much less defined than TAE version (suprising, was expecting cleaner bands).




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