User:Tk/Notebook/MF-xfm/2008/04/17

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Transformation results

  • seven colonies on the Monday 4/14 plates
    • all on low or medium concentration transformations

cat sequence from pKV104

  • pC194 sequence located at 974-2008 (comp) of genbank V01277
    • corresponds to pKV104 sequences 1975-3010
    • this includes all of cat gene with a portion of both flanking pC194 genes
      1  GATAAAGTCC GTATAATTGT GTAAAAGTcc cggggatccA AAAAGGCCAT 
     51  ATAACAGTCC TTTTACGGTA CAATGTTTTT AACGACAAAA ACATACCCAG 
    101  GAGGACTTTT ACATGACCCA AGTACATTTT ACACTGAAAA GCGAAGAGAT 
    151  TCAAAGCATT ATTGAATATT CTGTAAAGGA TGACGTTTCT AAAAATATTT 
    201  TAACAACGGT ATTTAATCAA CTAATGGAAA ATCAACGAAC AGAATATATT 
    251  CAAGCAAAAG AATATGAACG AACAGAAAAC CGACAAAGTC AACGAAATGG 
    301  CTATTATGAG CGCAGCTTTA CGACACGTGT AGGCACGCTA GAATTAAAAG 
    351  TACCCAGAAC ACGTGATGGC CATTTTTCAC CCACAGTGTT TGAACGTTAT 
    401  CAACGAAACG AAAAAGCCCT CATGGCTTCA ATGTTGGAAA TGTATGTATC 
    451  AGGCGTTTCA ACTCGTAAAG TATCAAAAAT TGTGGAAGAA CTTTGTGGTA 
    501  AATCCGTCTC TAAGTCCTTC GTTTCTAGCT TAACAGAACA GCTAGAACCT 
    551  ATGGTTAACG AGTGGCAGAA TCGTTTATTA TCAGAAAAAA ATTATCCTTA 
    601  CTTAATGACC GATGTACTCT ATATAAAAGT ACGAGAAGAA AATCGAGTAC 
    651  TCTCAAAAAG CTGTCATATA GCGATTGGAA TAACCAAAGA TGGCGACCGT 
    701  GAAATTATCG GCTTCATGAT TCAAAGTGGC GAAAGCGAAG AGACCTGGAC 
    751  AACATTTTTT GAATACCTAA AAGAACGCGG TTTACAAGGT ACGGAACTCG 
    801  TTATTTCTGA TGCGCACAAA GGATTAGTCT CTGCCATTAG AAAATCCTTC 
    851  ACCAACGTAA GTTGGCAAAG ATGCCAAGTT CACTTCCTAA GAAATATCTT 
    901  TACCACCATT CCTAAAAAAA ATTCAAAATC TTTCAGAGAA GCTGTTAAAG 
    951  GAATTTTTAA GTTCACAGAT ATTAACTTAG CGCGTGAGGC TAAAAATCGA 
   1001  TTGATTCATG ATTATATCGA TCAACCAAAA TATTCAAAAG CTTGCGCATC 
   1051  ATTGGATGAT GGATTCGAAG ACGCCTTTCA ATATACCGTA CAAGGAAATT 
   1101  CCCACAATCG ACTAAAGAGT ACCAATCTAA TTGAACGACT GAATCAAGAA 
   1151  GTACGCAGAA GAGAAAAGAT TATTCGCATC TTCCCCAATC AAACATCAGC 
   1201  CAATCGCTTA ATTGGAGCCG TTCTTATGGA CCTACATGAT GAATGGATTT 
   1251  ATTCTTCAAG AAAATACATC AATTTTGATA AGTAGAAATG GTAAAAACAT 
   1301  TGTATAGCAT TTTACACAGG AGTCTGGACT TGACTCACTT CCTTTATTAT 
   1351  TTTTCATTTT TTTAAACTAT TTAATACTAA TGTCTTTTAT AATAGCTTTT 
   1401  CATATATTAT ATAATCAATC TTTATAAGTC CTTTTATAAA TTTCTTTTCT 
   1451  ACCATTTTCG ATAAATTCCT GTTTAATGGG GATCCGTCGA CGCTCTCCCT 
   1501  TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG 
   1551  AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG 
   1601  TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA 
   1651  TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA 
   1701  TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG 
   1751  TCCGGCGTAG AGGATCAATC TTCATCCATT CCAAGGTAAA TCCCCCTTCG 
   1801  CCGTTTCTGT TACCATTATA CACCTTTTGA ACCTTAACGT AAACGTTAAG 
   1851  TTTTAAAAAA CAATAAAAAA GACGAGCAGC ATACAGCACC CGTCTTTCAC 
   1901  TTTCCTGTTT AAGCTAAACT TCCCGCCACT GACAGAGACT CTTTTTGAAG 
   1951  GCTTTCAGAA AGCACTCGAT ACGCGATCTG GAGCTGTAAT ATAAAAACCT 
   2001  TCTTCAACTA ACGGGGCAGG TTAGTGACAT TAGAAAACCG ACTGTAAAAA 
   2051  GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT 
   2101  GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA 
   2151  AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT 
   2201  TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA 
   2251  TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA 
   2301  AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG 
   2351  AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG 
   2401  AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC 
   2451  ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC 
   2501  CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT 
   2551  GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT 
   2601  GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG 
   2651  TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT 
   2701  AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA 
   2751  AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC 
   2801  TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT 
   2851  CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA 
   2901  GAATAAAAGA CCACATTAAA AAATGTGGTC TTTTGTGTTT TTTTAAAGGA 
   2951  TTTGAGCGTA GCGAAAAATC CTTTTCTTTC TTATCTTGAT AATAAGGGTA 
   3001  ACTATTGCCG ATGATAAGCT GTCAAACATG AGAATTCCCA TTCCTTAATA 
   3051  AAAGATAATA ATTTTGAACC ATAACCTTTG TCTTGATAGT TAGGTAACAT 
   3101  TCCTATTGAG TGTAAATAAA ACATTGTTTT TCCATCAGGT CTTAAAAGTG 
   3151  TATAGCAATA TGCAAATCCT ATAATTTTAT TATTTTCTTT AGCTATAAAC 
   3201  CCAAATGAAC TGGTATCATT AAGAAATCCT TTTAAATTAT CAATATCAAA 
   3251  AACCATATTA TCATCAATTA AAACTTCTTC CATAAACTCA GTCAAGTCCA 
   3301  GACTCCTGTG TAAAATGCTA TACAATGTTT TTACCATTTC TACTTATCAA 
   3351  AATTGATGTA TTTTCTTGAA GAATAAATCC ATTCATCATG TAGGTCCATA 
   3401  AGAACGGCTC CAATTAAGCG ATTGGCTGAT GTTTGATTGG GGAAGATGCG 
   3451  AATAATCTTT TCTCTTCTGC GTACTTCTTG ATTCAGTCGT TCAATTAGAT 
   3501  TGGTACTCTT TAGTCGATTG TGGGAATTTC CTTGTACGGT ATATTGAAAG 
   3551  GCGTCTTCGA ATCCATCATC CAATGATGCG CAAGCTTTTG AATATTTTGG 
   3601  TTGATCGATA TAATCATGAA TCAATCGATT TTTAGCCTCA CGCGCTAAGT 
   3651  TAATATCTGT GAACTTAAAA ATTCCTTTAA CAGCTTCTCT GAAAGATTTT 
   3701  GAATTTTTTT TAGGAATGGT GGTAAAGATA TTTCTTAGGA AGTGAACTTG 
   3751  GCATCTTTGC CAACTTACGT TGGTGAAGGA TTTTCTAATG GCAGAGACTA 
   3801  ATCCTTTGTG CGCATCAGAA ATAACGAGTT CCGTACCTTG TAAACCGCGT 
   3851  TCTTTTAGGT ATTCAAAAAA TGTTGTCCAG GTCTCTTCGC TTTCGCCACT 
   3901  TTGAATCATG AAGCCGATAA TTTCACGGTC GCCATCTTTG GTTATTCCAA 
   3951  TCGCTATATG ACAGCTTTTT GAGAGTACTC GATTTTCTTC TCGTACTTTT 
   4001  ATATAGAGTA CATCGGTCAT TAAGTAAGGA TAATTTTTTT CTGATAATAA 
   4051  ACGATTCTGC CACTCGTTAA CCATAGGTTC TAGCTGTTCT GTTAAGCTAG 
   4101  AAACGAAGGA CTTAGAGACG GATTTACCAC AAAGTTCTTC CACAATTTTT 
   4151  GATACTTTAC GAGTTGAAAC GCCTGATACA TACATTTCCA ACATTGAAGC 
   4201  CATGAGGGCT TTTTCGTTTC GTTGATAACG TTCAAACACT GTGGGTGAAA 
   4251  AATGGCCATC ACGTGTTCTG GGTACTTTTA ATTCTAGCGT GCCTACACGT 
   4301  GTCGTAAAGC TGCGCTCATA ATAGCCATTT CGTTGACTTT GTCGGTTTTC 
   4351  TGTTCGTTCA TATTCTTTTG CTTGAATATA TTCTGTTCGT TGATTTTCCA 
   4401  TTAGTTGATT AAATACCGTT GTTAAAATAT TTTTAGAAAC GTCATCCTTT 
   4451  ACAGAATATT CAATAATGCT TTGAATCTCT TCGCTTTTCA GTGTAAAATG 
   4501  TACTTGGGTC ATGTAAAAGT CCTCCTGGGT ATGTTTTTGT CGTTAAAAAC 
   4551  ATTGTACCGT AAAAGGACTG TTATATGGCC TTTTTACTTT TACACAATTA 
   4601  TACGGACTTT ATCTACATCA GAAAGACTTA CGACTCCTGT AATGATAGCA 
   4651  ACAACCGCTC CAATTAAAGC TGTAATACCA ATATCTAAAC CTTTTGGTTG 
   4701  CCAAATCACA AAGGTTAAAG TTAAAAGAAA AATTACAATT GCTAAAATAG 
   4751  TCATCAACAG TCACCTGATT TCACATTTTT ACATACACAT CTTTGATTAG 
   4801  ATGTATTAAT GATGTTCAAG TTTTGGATAA TATCATCTAA AATAGCATGA 
   4851  TTAAGTTGAT ACCAATGTTT ATTGCCATCT TTTCGTGTTG TAACTAATTC 
   4901  ATTATCTACT AATGACTTCA TATGATGACT TAGTGTAGGT TGTGAGAATT 
   4951  GAAAGTGTTC TAATAAGTCA CAAGCGCATA GCTCACCACA AGAAAGTAAA 
   5001  TCTAATATTT CTAACCTACT TGGATCTGAT AAAATTTTTA ATATTGTTGA 
   5051  TAGTTCTTTA TAAGACATGA ATAAACCTCC TAGATATTAA ATAGATTAAC 
   5101  ATCTATATAG ATTATTGTCT ATGTTATTTT ATTTACTATT TTTTATTCTA 
   5151  TATATAGTTT GACGTGTAAT ACCAACTTCT TTAGCTATAG TACTTATAGA 
   5201  TTTACCCTGT TCAAGTAATT CAACAACTCG GTAATAAACT AAACGCTTTT 
   5251  GTGGGTCTTT AGCATTGGGA GAATACAGAA CAGGTCTCCC TTTATATATA 
   5301  CCTTTTTCTT TGGCGACTTG AATCCCTTGA GCTTGTCGAC CTCGAGGGGG 
   5351  GGCCCGGTAC CCAATTCGCC CTATAGTGAG TCGTATTACA ATTCACTGGC 
   5401  CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 
   5451  ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 
   5501  GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 
   5551  GAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA 
   5601  AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA 
   5651  AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC 
   5701  AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC 
   5751  CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT 
   5801  TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC 
   5851  CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA 
   5901  AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG 
   5951  TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG 
   6001  GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 
   6051  TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 
   6101  CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 
   6151  CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 
   6201  TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 
   6251  TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 
   6301  CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 
   6351  AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 
   6401  AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 
   6451  CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 
   6501  CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 
   6551  CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 
   6601  GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 
   6651  ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 
   6701  TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 
   6751  TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 
   6801  GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 
   6851  GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 
   6901  CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 
   6951  AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 
   7001  TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 
   7051  TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 
   7101  CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 
   7151  AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 
   7201  TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 
   7251  TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 
   7301  AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 
   7351  AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 
   7401  AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 
   7451  GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 
   7501  TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 
   7551  ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 
   7601  ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 
   7651  CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 
   7701  CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 
   7751  TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 
   7801  TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 
   7851  TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 
   7901  CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA 
   7951  CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 
   8001  CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG 
   8051  TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 
   8101  GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 
   8151  CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG 
   8201  AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG GGCTGCAGAA 
   8251  ACGATATTAA CTAAGTTACT TACAATTAAG GGAAGTGATG TAGTATCAGC 
   8301  AATAAAACCA CTGGCAATAA TAAAGGGGAA AATCACTTTT TGATTAAATC 
   8351  CTAGATTCCT TACCATCGCT AATACAATAG GCGTTAAGAT TAAAGCTGCA 
   8401  CCATCATTTG CGAAAAATGC TGCTACAATT GCCCCAAGTA ACATAATAAA 
   8451  AACAAACATT TTTAAGCCAT TACCGTTTGA AGCCTTGACC ATATGTATCG 
   8501  CAGACCATTC AAAAAAACCA ATTTCATCTA ATATTAATGA AATAAGAATA 
   8551  ACAGCCACAA AAGTTAAAGT TGCATTCCAA ACAATACCTG TTACTTCTAA 


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