User:Tk/Notebook/MF/2009/10/01

From OpenWetWare

Jump to: navigation, search
Mesoplasma florum simplification Main Page
Previous entry      Next entry

PCR of pWV01 origin

  • construction of registry J70500 part
  • PCR from
    • pMG36 colony 1 (Kan resistant, no plasmid?)
    • pMG36E colony 1 (Ery resistant)
    • pMG36C colony 1 (Cm resistant)
    • pMG36 colony 2 (Kan resistant, no plasmid)
    • pMG36E colony 2 (Ery resistant)
  • Prep of PCR from Ery resistant bands gives 67 and 89 ng/ul in 50 ul volume


Personal tools