User:Tk/Notebook/MF/2009/11/08

Resequencing L1, PP, GF, W12

• Growth after 24 hours showed differences in colony diameter
  • L1: 6-7 divisions about 100 microns
  • PP: 4-5 divisions about 75 microns
  • GF: 2-3 divisions about 40 microns
  • W12: no visible colonies, possible contamination with yeasts
  • sterile control shows no growth

• Scale is 16.5 microns/division

• pick single colonies of L1, PP, GF into two 10 ml cultures
• Streak out 3/25/09 culture of W12

Annotation

• M. pneumoniae phosphatase mpn248 is required for tip assembly, PMID
• M. florum has genes mfl220 and mfl221 homologous to mpn247 and mpn248
• M. florum genes are dispensable