User:Tk/Notebook/MF/2009/11/22

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Mesoplasma florum simplification Main Page
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Cutting DNA with Sau3AI and SpeI

Cut 1 μg DNA in 50 μl in buffer 2, BSA, 1 μl enzyme


  • L1
    • Uncut
    • Sau3AI digest
    • SpeI digest
  • GF
    • Uncut
    • Sau3AI digest
    • SpeI digest
  • PP
    • Uncut
    • Sau3AI digest
  • Ent
    • Uncut
  • PP
    • SpeI
  • Ent
    • SpeI digest
    • Sau3AI digest


L1 cannot be cut with Sau3AI. Poorly cut with SpeI. Other strains are completely cut with Sau3AI, cut similarly with SpeI.



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