User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/04/15

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Objective

  1. Test fluorescence of nanoparticles
  2. Microscope Analysis
  3. Preparation of sonicated samples

Microscope Analysis

Please refer to Puja Mody's Open Wetware Notebook for microscope analysis of samples

Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Image: Screen_Shot_2014-05-07_at_4.12.08_AM.png

Table 2: FeSO4 Stock Prep

Image: Screen_Shot_2014-05-07_at_4.16.17_AM.png

Table 3: Hemoglobin Prep

Image:Screen Shot 2014-05-07 at 4.12.28 AM.png

Table 4: KNO3 Prep

Image: Screen_Shot_2014-05-07_at_4.12.36_AM.png

Table 5: KOH Prep

Image: Screen_Shot_2014-05-07_at_4.12.43_AM.png

Table 6: Gold Prep

Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png

Table 7: Magnetite/ BSA Prep

Image: Screen_Shot_2014-05-07_at_4.13.08_AM.png

Table 8: Magnetite/ Hemoglobin Prep

Image:Screen_Shot_2014-05-07_at_4.13.19_AM.png

Table 9: Gold added to Magnetite/ BSA Prep

image: Screen_Shot_2014-05-07_at_4.13.25_AM.png

Table 10: Gold added to Magnetite/ Hemoglobin Prep

Image: Screen_Shot_2014-05-07_at_4.13.32_AM.png

Atomic Absorption

Table 1: Preparation of iron standard used fro AA

Image: Screen_Shot_2014-05-07_at_4.33.45_AM.png

Fluorescence Testing

Procedure

  1. Centrifuge 1.5 eppendorf tube with dye samples for 15 minutes
  2. Use micropipette to remove supernatant
  3. Add 1.3 mL of deionized water to sample
  4. Remove deionized water from sample
  5. Repeat steps 3 and 4 three times
  6. Place sample over UV light (fluorescence should be seen within the hydrogels)
  7. Vortex sample for 2 minutes
  8. Apply magnetic field to sample
  9. Repeat steps 7 and 8 continuously for approximately 4 minutes
  10. Test the deionized water for fluorescence


Table 1: Excitation and Emission Fluorescence Spectra of Schlenk Line, Sonicator, and Oven methods

Image: Screen_Shot_2014-05-07_at_4.36.53_AM.png

The sonicated hydrogels gave the highest intensities of all preparation methods


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