User:Tyson R. Shepherd

Contact Info

Tyson R. Shepherd

• Tyson R. Shepherd
• Uppsala University
• Uppsala, Sweden
• Email me through OpenWetWare

I am a postdoctoral fellow in Prof. Anthony Forster's lab at Uppsala University.

Education

• 2011, PhD in Biochemistry, University of Iowa, Iowa City, USA (Ernesto Fuentes Lab)
• 2005, MS in Physics, Georgia Institute of Technology, Atlanta, USA (Predrag Cvitanovic Lab)
• 2003, BS in Physics, Illinois State University, Normal, USA (Brian Clark Lab)

Research interests

1. In vitro translation in synthetic biology
2. Multi-gene construct design and assembly
3. Molecular scaffolds

Publications

1. Shepherd TR, Klaus SM, Liu X, Ramaswamy S, DeMali KA, and Fuentes EJ. The Tiam1 PDZ domain couples to Syndecan1 and promotes cell-matrix adhesion. J Mol Biol. 2010 May 21;398(5):730-46. DOI:10.1016/j.jmb.2010.03.047 |

   The Tiam1 PDZ domain couples to Syndecan1 and promotes cell-matrix adhesion

2. Shepherd TR, Hard RL, Murray AM, Pei D, and Fuentes EJ. Distinct ligand specificity of the Tiam1 and Tiam2 PDZ domains. Biochemistry. 2011 Mar 1;50(8):1296-308. DOI:10.1021/bi1013613 |

   Distinct ligand specificity of the Tiam1 and Tiam2 PDZ domains

3. Punekar AS, Shepherd TR, Liljeruhm J, Forster AC, and Selmer M. Crystal structure of RlmM, the 2'O-ribose methyltransferase for C2498 of Escherichia coli 23S rRNA. Nucleic Acids Res. 2012 Nov 1;40(20):10507-20. DOI:10.1093/nar/gks727 |

   Crystal structure of RlmM, the 2'O-ribose methyltransferase for C2498 of Escherichia coli 23S rRNA

4. Liu X, Shepherd TR, Murray AM, Xu Z, and Fuentes EJ. The structure of the Tiam1 PDZ domain/ phospho-syndecan1 complex reveals a ligand conformation that modulates protein dynamics. Structure. 2013 Mar 5;21(3):342-54. DOI:10.1016/j.str.2013.01.004 |

   The structure of the Tiam1 PDZ domain/ phospho-syndecan1 complex reveals a ligand conformation that modulates protein dynamics

5. Liu Y, Collins C, Kiosses WB, Murray AM, Joshi M, Shepherd TR, Fuentes EJ, and Tzima E. A novel pathway spatiotemporally activates Rac1 and redox signaling in response to fluid shear stress. J Cell Biol. 2013 Jun 10;201(6):863-73. DOI:10.1083/jcb.201207115 |

   A novel pathway spatiotemporally activates Rac1 and redox signaling in response to fluid shear stress

6. Punekar AS, Liljeruhm J, Shepherd TR, Forster AC, and Selmer M. Structural and functional insights into the molecular mechanism of rRNA m6A methyltransferase RlmJ. Nucleic Acids Res. 2013 Nov;41(20):9537-48. DOI:10.1093/nar/gkt719 |

   Structural and functional insights into the molecular mechanism of rRNA m6A methyltransferase RlmJ

7. Shepherd TR and Fuentes EJ. Structural and thermodynamic analysis of PDZ-ligand interactions. Methods Enzymol. 2011;488:81-100. DOI:10.1016/B978-0-12-381268-1.00004-5 |

   Review: Structural and thermodynamic analysis of PDZ-ligand interactions

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