User:Vijay/Notebook/2007-2-12

From OpenWetWare

Jump to: navigation, search

Fusion

  1. Plasmid prep from phoA/lacZ (pBAF/pLKC480) and gel. Done
  2. Double digest phoA/lacZ and gel. Done
    1. 8 μl vector
    2. 2 μl buffer
    3. 1 μl enzyme
      • EcoR1 for 1hr at 370C, followed by Sal1 for 1hr at 370C in cycler
    4. 9 μl water
  3. Stock phoA/lacZ. Done


Fractionation

  1. Inoculate UAMS-1 wt for 2nd fractionation. Done
Personal tools