User:Wesley J. Houston/Notebook/MAP/2013/03/22

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  • Gel purification was performed using the KAH201 plasmids prepped on 2/28, as well as the KAH182 plasmids prepped on 3/21.
    • No DNA was present in the second lane, and the concentrations and purities were determined to be too low to complete a ligation.
  • Grew more KAH201 liquid cultures.
    • Placed colonies in LB broth overnight in shaking 37C incubator.
Reagent Volume   Results of third electrophoresis
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample 260/280 ng/μL
1. KAH201 (S/P) 0.32 0.851
2. KAH182 (X/P) 0.385 1.064

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