3/26/13
- Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
- Determined yields of plasmids.
Sample |
OD260 |
260/280 |
ng/μL
|
1. KAH201 (Plasmid) |
0.056 |
1.732 |
56.395
|
2. KAH201 (Plasmid) |
0.069 |
1.857 |
69.164
|
Reagent |
Volume |
|
30 μL/lane, 1% agarose; Ladder
|
DNA (plasmid) |
25 μL
|
10x buffer |
3.0
|
enzyme 1 (Pst1) |
1.0
|
enzyme 2 (Xba1 or Spe1) |
1.0
|
dH2O |
0.0 μL
|
|
30 μL --> 37°C/ ~15 min.
|
> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)
Sample |
OD260 |
260/280 |
ng/μL
|
1. KAH201 (S/P) |
0.029 |
1.054 |
29.262
|
2. KAH201 (S/P) |
0.006 |
1.381 |
6.172
|
3. KAH182 (X/P) |
-0.002 |
2 |
-1.702
|
4. KAH182 (X/P) |
0.007 |
5.583 |
7.129
|
|