User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/18

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RII Overlay & Stop stimulation ELISA Main project page
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Summary

  • RII overlay for blot made 09May2010
  • Stop stimulation and collect samples for ELISA
    • Substrate buffer

Notes

  • Due to a missing filter the machine planned to use for viability staining a new machine had to be used and due to this more time had passed, plates were stored at 4 °C in the mean time.
  • Fluorescence was not measurable anymore, absorbance was used instead.

Results

  • ALAMAR BLUE Viability staining see 19May2010

Discussion

  • Alamar blue seems to work fine with only absorbance


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