- ELISA (Caro (12:08) & Mine (11:55))
- Mine was the same as the previous (01August2010)
- NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
| || ||1||2||3||4||5||6
| CTR||A||CTR ||CTR+8P ||CTR+6Bnz ||CSE+Ht31 ||DMSO+CTR ||Cotransin+CTR (2/400)
| CTR||B||CTR ||CTR+8P ||CTR+6Bnz ||CSE+Ht31 ||DMSO+CTR ||Cotransin+CTR (6/400)
| CSE||C||CSE ||CSE+8P ||CSE+6Bnz ||CSE+Ht31+6Bnz ||DMSO+CSE ||Cotransin+CSE (2/400)
| CSE||D||CSE ||CSE+8P ||CSE+6Bnz ||CSE+Ht31+6Bnz ||DMSO+CSE ||Cotransin+CSE (6/400)
- New cells were plated out, D9 P23
- RII Overlay showed NOTHING!! Not even marker signals...
- Most probable cause, blot was incubated ON up side down?
- IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on