User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/12

From OpenWetWare

Jump to: navigation, search

Lyse HEK293 (isolate RNA), 2nd AB WB, Convocal microscopy Main project page
Previous entry      Next entry

Summary

  • 2nd Antibody (mouse) on WB, 1:1000 dilution (SHOULD BE 1:10.000)
  • RIPA (Lysis) Buffer (per 1 mL)
    • 1.06 mg β-Glycerolphosphate
    • 1 mL RIPA buffer
    • 8 μL Protease inhibitor mix
    • 1 μL NaF (1 M)
    • 10 μL NaVO3 (10 mM)
    • 12.5 μL PMSF
  • Lysate HEK293 Cells
  • CONVOCAL MICROSCOPY!
  • TODO Isolate RNA of HEK293 Cells

Results

  • Western blot

  • Quick preview of IF results
    • No good picture of Gravin localisation.
    • And no changes in RII with Ht31

Discussion

  • DONE Ow..

Attachment

RNA concentration HEK293


Personal tools