User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/09/22

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Summary

  • Dilution of samples to 20 μg/30 μL (5x) + 10 μL LB per 30 μL sample
' μL μL
SampleddH2O
Jurkat25,0125,0
HEK293 (12/08/2010)25,4124,6
HeLa -S (p17 (20.6.2010)10,7139,3
S0 (7.5.2010)97,952,1
S121,5128,5
S262,088,0
S322,2127,8
C0 (7.5.2010)150,00,0
C124,6125,4
C214,9135,1
C343,9106,1
  • Run 1x 8% SDS-PAGE
8% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Jurkat
(30 μL)
HEK293
(30 μL)
HeLa -S
(30 μL)
Marker
(5 μL)
S0
(30 μL)
S1
(30 μL)
S2
(30 μL)
S3
(30 μL)
Marker
(5 μL)
C0
(30 μL)
C1
(30 μL)
C2
(30 μL)
C3
(30 μL)
X
  • Run at 200 V for 40 min.


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