Summary
- Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
- Preparation of 2 10% Tris-glycine acrylamide gels
- Dilution of samples, corrected to lowest concentration
| Sample
| μL Sample
| μL dH2O
|
| HEK293 (12/08/2010) | 61,5 | 88,5
|
| HeLa -S (p17 (20.6.2010) | 26,0 | 124,0
|
| S0 (7.5.2010) | 236,9 | 0,0
|
| S1 | 52,1 | 97,9
|
| S2 | 150,0 | 0,0
|
| S3 | 53,8 | 96,2
|
| C0 (7.5.2010) | 2470,4 | 0,0
|
| C1 | 59,6 | 90,4
|
| C2 | 36,1 | 113,9
|
| C3 | 106,3 | 43,7
|
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
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| 1
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|
Gel
Lysates
| X
| Fermantas high range Marker
(5 μL)
| HEK293
(30 μL)
| HeLa -S
(30 μL)
| Jurkat
(30 μL)
| Fermantas high range Marker
(10 μL)
| S0
(30 μL)
| S1
(30 μL)
| S2
(30 μL)
| S3
(30 μL)
| C0
(30 μL)
| C1
(30 μL)
| C2
(30 μL)
| C3
(30 μL)
| X
|
- Run at 200 V
- 10% gel was cut in 4 for
- GAPDH (1:2000, mouse (<40kDa))
- RIIα (1:1000, rabbit (40 - 75kDa))
- AKAP79 (1:1000, rabbit? (75 - 130kDa)) (Santa Cruz)
- AKAP250 (1:1000, Goat, (>130kDA)) (Santa Cruz)
|
Project name
Main project page
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