User:Xiling Shen/Notebook/Lab book

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. Digest (small) BglII/AlwNI

RXN MIX FOR 1 SAMPLES B2Ms 4 EcoRI 0.5 AlwN1 0.5


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW358 Keep: 1000 Reject: 2796


Image:GelExample.jpg . Digest (small) BamHI/AlwNI

RXN MIX FOR 1 SAMPLES B2Ms 4 BamHI 0.5 AlwNI 0.5


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW913 Keep: 3500 Reject: 931


Image:GelExample.jpg . Digest (small) EcoRI/NheI

RXN MIX FOR 1 SAMPLES B2Ms 4 EcoR1 0.5 NheI 0.5


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW935 Keep: 7462 Reject: 918


Image:GelExample.jpg . Digest (small) EcoRI/XbaI/Bgl1

RXN MIX FOR 1 SAMPLES B2Ms 4 EcoRI 0.5 XbaI 0.5


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW914 Keep: 2453 Reject: 1134/914


Image:GelExample.jpg . Digest (small) XbaI/PstI

RXN MIX FOR 1 SAMPLES B2Ms 4 XbaI 0.5 PstI 0.5


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW916 Keep: 8274 Reject: 1190


Image:GelExample.jpg . Digest (small) SpeI/PstI/AlwN1

RXN MIX FOR 1 SAMPLES B2Ms 4 SpeI 0.3 PstI 0.3 AlwN1 0.3


Aliquote 5uL rxn mix to 1 tubes and add 5uL of each DNA sample (below) to the digests Incubate reaction at 37oC for 1 - 2.5 hours (start @ ___, end @ ___ ) Gel purify bands indicated below by "Keep"

DNA: pWW937 Keep: 4878 Reject: 2578,1350,427,263


Image:GelExample.jpg .

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