User:Yoonseok Jung/Notebook/Polycomb Project/2011/02/28

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  • DNA Digest
 Insert(5XGAL4) | Vector(Spacer)
 DNA    25uL    | DNA     10uL   
 10X FD  3uL    | 10X FD   3uL
 EcoRI   1uL    | EcoRI    1uL
 SpeI    1uL    | XbaI     1uL
dH2O 15uL
        (30uL)  |        (30uL)
 37C/15min
  • Gel synthesis
 1. 1X TAE 60mL + 0.7g Agarose
 2. 40s microwave, shake a flake, and 40s microwave again
 3. Add 6uL EtBr



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