[TetR][rbs][LuxR][dblTerm][LuxPR]+[rbs][LacI][dblTerm] digest pSB1A2-F2620 (Spe/BglI, Neb2, Large-1998) digest pSB1A2-P0412 (Xba/BglI, Neb2, Large-2458) ligate, Product is pSB1A2-J23040