WangLab:Ligation Samples

From OpenWetWare

Jump to: navigation, search

Ligation Samples

Two piece ligation

  • 5X ligation buffer: 4
  • Vector: 1
  • Insert: 3
  • T4 ligase: 1
  • H2O: 11

Three piece ligation

  • 5X ligation buffer: 4
  • Vector: 1
  • Insert 1: 5
  • Insert 2: 5
  • T4 ligase: 1
  • H2O: 4
Personal tools