WangLab:Lysis Buffer

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Lysis Buffer (Non-strigent)

MQ water 13.65 ml
2M Tris/Hcl (ph7.5) 375 ul
5M NaCl 375 ul
Triton X-100 150 ul
0.1% SDS 150 ul
0.5M NaF 150 ul
100 mM Na3VO4 150 ul
100 mM PMSF 150 ul
10 mg/ml Leupeptin 15 ul
SDS Gel-loading buffer: 4X 6X
Tris.Cl (PH 6.8) (mM 200 300
Dithiothreltol (DTT, mM) 400 600
SDS (Electrophoresis 8% 12%
Bromophenol Blue 0.4% 0.6%
Glycerol 40% 60%
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