# WinterVomitingLab:Notes/Virus weight calculations

### From OpenWetWare

What is the weight of human norovirus? Or VLPs?

MW of ssRNA genome

= (7.5kb x 320.5) + 159.0 = 2,404 kDa (or g/ml) = 3.99 x 10^-12 ug

VP1 capsid of 90 dimers (180 copies)

= 60 kDa x 180 = 10,800 kDa = 1.8 x 10^-11 ug

VP2 (estimated 3 copies per capsid)

= 22 kDa x 3 = 66 kDa

VPg (one copy per genome)

= 15.8 kDa

For VLPs consisting of only VP1, see above.

For viruses:

= 2,404 + 10,800 + 66 + 15.8 = 13,286 KDa = 2.2 x 10^-11 ug

Therefore, for a 1 ug/ml preparation of VLP,

= 1 / (1.8 x 10^-11) = 5.6 x 10^10 viruses / ml

For a virus stock that contains 1 x 10^7 viruses / ml

= (1 x 10^7 viruses) x (2.2 x 10^-11 ug) = 0.00022 ug of viruses / ml