Yu:CBB
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1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker | 25mM Tris-HCl |
2. Add 15mL of 5M NaCl | 150mM NaCl |
3. Add 500 μL of 1M Magnesium Acetate | 1mM MgOAc |
4. Add 500 μL of 1M Imidazole | 1mM Imidazole |
5. Add 1 mL 1M CaCl2 | 2mM CaCl2 |
5. Bring volume to 500mL with millipore H2O in a graduated cylinder | Volume to 500mL |
6. Complete immediately prior to use with 0.696 μL β-mercaptoethanol per 1 mL CBB | 10mM β-mercaptoethanol |
7. Keep completed CBB on ice at all times |