Yu:NP40 Buffer
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1. Measure 500 mL millipore H2O into a 1L beaker | |
2. Add 30 mL 0.5M Na2HPO4 (Sodium phosphate dibasic) | 15 mM Na2HPO4 |
3. Add 20mL 0.5M NaH2PO4 (Sodium phosphate monobasic) | 10 mM NaH2PO4 |
4. Add 100mL 10% NP-40 (Igepal CA-630 or Nonidet P-40) | 1% NP-40 |
5. Add 30mL 5M NaCl | 150 mM NaCl |
6. Add 4mL 0.5M EDTA | 2 mM EDTA |
7. Add 2.0995 g NaF | 50 mM NaF |
8. Add 1mL 100 mM Na3VO4 | 0.1 mM Na3VO4 |
9. Bring volume to mL total with millipore H2O in a graduated cylinder | Bring volume to 1L |