Yu:SDS PAGE Sample Buffer
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1. Measure 2mL glycerol into a 15mL tube | 2mL glycerol |
2. Add 4mL 10% SDS | 4mL 10% SDS |
3. Add .3g dithiothreitol (DTT) | .3g DTT |
4. Add a small amount of bromophenol blue | Pinch of bromophenol blue |
5. Aliquot 1mL into eppendorf tubes and store in a -20°C freezer | Aliquot and store |