Yu:TES

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TES

Final Concentration For 50 mL For 100 mL
10 mM Tris pH 8 0.5 mL of 1M Tris pH 8 1 mL of 1M Tris pH 8
10mM EDTA pH 8.0 1 mL of 0.5 M EDTA pH 8.0 2 mL of 0.5 M EDTA pH 8.0
0.5% SDS 1.25 mL of 20% SDS 2.5 mL of 20% SDS
RNase-free H2O 47.25 mL 94.5 mL
All reagents must be RNase-free
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