Yu:southern sol
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Alkaline Denaturation Solution
Final Concentration | For 1 Liter | For 4 Liters |
1.5 M NaCl | 87.7.3 g | 351 g |
0.5 M NaOH | 20 g | 80 g |
milliQ H2O | to 1 L | to 4 L |
Neutralization Solution
Final Concentration | For 1 Liter | For 4 Liters |
1.5 M NaCl | 87.7 g | 351 g |
0.5 M Tris Base | 60 g | 240 g |
Adjust pH to 7.5 with HCl | ||
milliQ H2O | to 1 L | to 4 L |
Pre-hybridization Buffer
Final Concentration | For 100 mL | For 250 mL |
6x SSC | 30 mL of 20x SSC | 75 mL of 20x SSC |
2.5x Denhardt's | 5 mL of 50x Denhardt's | 12.5 mL of 50x Denhardt's |
0.5% SDS | 2.5 mL of 20% SDS | 6.25 mL of 20% SDS |
milliQ H2O | to 100 mL | to 250 mL |
Store at RT or 4°C | ||
Warm up the solution prior to use to redissolve the SDS |
Hybridization Buffer
Final Concentration | For 100 mL | For 250 mL |
6x SSC | 30 mL of 20x SSC | 75 mL of 20x SSC |
1x Denhardt's | 2 mL of 50x Denhardt's | 3 mL of 50x Denhardt's |
0.1% SDS | 0.5 mL of 20% SDS | 1.25 mL of 20% SDS |
milliQ H2O | to 100 mL | to 250 mL |
Store at RT or 4°C | ||
Warm up the solution prior to use to redissolve the SDS |