UA Biophysics:Protocols

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Contents

  • 1 ANTIBIOTICS STOCK
  • 2 BACTERIA STOCK
  • 3 BUFFERS
  • 4 CHEMICALS
  • 5 EXTRACTION
  • 6 MEDIA
  • 7 MICROFLUIDICS
  • 8 MOLECULAR BIOLOGY
  • 9 PLASMIDS AND RELATED
  • 10 PHAGES
  • 11 PREPARACIÓN DE LUV Y GUV
  • 12 TURBIDOSDOSTATS

ANTIBIOTICS STOCK

• Ampicillin
• Kanamycin
• Chloramphenicol

BACTERIA STOCK

• Stocks

BUFFERS

• PBS

CHEMICALS

• KOH

EXTRACTION

• Lipid Extration
• Carotenoid Extration

MEDIA

• M9 Salts (5x) 1L
• M9 (5x) 1L
• LB 1L
• LB Plates 1L
• SD 1L
• 1M MgSO4
• 0.1M CaCl2

MICROFLUIDICS

• Soft lithography
• Master fabrication
• Plasma Cleaner Protocol
• Cell Insertion Mother Machine
• Microfluidics Valves

MOLECULAR BIOLOGY

• Q5 Polymerase

• Phusion Polymerase

PLASMIDS AND RELATED

• GenEluteTM HP Plasmid Miniprep Kit
• Preparing Chemically Competent Cells
• Transforming Chemically Competent
• Competent Cells

PHAGES

• Marking T4 DNA with DAPI

• Direct Plating Plaque Assay

• Small Drop Plaque Assay System

PREPARACIÓN DE LUV Y GUV

• LUV

• GUV

TURBIDOSDOSTATS

• Plate turbidostat user manual
• Revolver turbidostat with interchanging media


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