Wikiomics:PSI-BLAST

PSI-BLAST - Position-Specific Iterated BLAST. Variant of BLAST used for disovery of weak but relevant protein sequence matches. The algorithm is based on running consequitive BLAST searches using position weight matices:

• query sequence ->
• first blast search ->
• first iteration blast results ->
• sequence alignment of strong matches ->
• creating a profile #1 ->
• second iteration blast search with profile #1 as a query ->
• second iteration blast results, etc.

PSI-BLAST server at NCBI: link

Tutorials

• @NCBI Part 1
• @NCBI Part 2
• @NCBI Part 3
• @Uni-Saaraland (DOC)]