IGEM:Peking University/2008/Experiment/PKU 0807014 G1 1: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
← Older edit
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
==PKU_080713_G1_1==
==PKU_080714_G1_1==
<hr class=divider>
<hr class=divider>
::'''Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control<sub>1</sub>/Control<sub>2</sub>/Control<sub>3</sub>)'''
::'''Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control<sub>1</sub>/Control<sub>2</sub>/Control<sub>3</sub>)'''
Line 43: Line 43:
[[Image:PKU_080714_lacS-tet-pGREG504cut.tif|center|400px]]
[[Image:PKU_080714_lacS-tet-pGREG504cut.tif|center|400px]]
|}
|}
/*''lanes:lacS|tet|vector|marker1|marker2''
::::::::::::'''lanes:lacS|tet|vector|marker1|marker2'''




Line 97: Line 97:
|-
|-
|ddH<sub>2</sub>O||12.6
|ddH<sub>2</sub>O||12.6
|}
{|align="right"
|back to [[PKU_Experiment|Previous Page]]
|}
|}

Latest revision as of 15:11, 17 July 2008

PKU_080714_G1_1

Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
Enzyme cutting-pADH-(lacS/tet)(PCR product)
Volume (μL)
pADH-(lacS/tet) 14
SacI 2
XhoI 2
M Buffer 10
ddH2O 72


Enzyme cutting-pGREG504-1
Volume (μL)
pGREG504-1 4
SacI 2
XhoI 2
M Buffer 10
ddH2O 82


lanes:lacS|tet|vector|marker1|marker2


Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3) 5
SacI 1
XhoI 1
M Buffer 2
ddH2O 11


Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2) 5
SphI 0.2
XhoI 0.2
H Buffer 2
ddH2O 12.6


Volume (μL)
pGREG504-pADH-(Control1/Control2/Control3) 5
BamHI 0.2
XhoI 0.2
K Buffer 2
ddH2O 12.6
back to Previous Page
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Experiment/PKU_0807014_G1_1&oldid=221675"