User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">ELISA & Counting splitting cells</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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===Method=== | ===Method=== | ||
====ELISA==== | |||
* Same actions as [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18|18Feb2010]] | |||
{| border="1" | {| border="1" | ||
|+''' | |+'''Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))''' | ||
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====Counting and splitting cells==== | |||
==Results== | ==Results== | ||
*{{todo| | *HT4 cells | ||
** 286 | |||
** 249 | |||
** 203 | |||
** 193 | |||
*D12 hTERT cells (15.75×10<sup>4</sup> cells/mL) | |||
** 20 | |||
** 12 | |||
** 15 | |||
** 16 | |||
** S<sup>+</sup> 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL) | |||
*D12 P28 of Mark hTERT cells (75.5×10<sup>4</sup> cells/mL) | |||
**92 | |||
**67 | |||
**83 | |||
**60 | |||
**0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells | |||
* D9 (53.5×10<sup>4</sup> cells/mL) | |||
** 52 | |||
**55 | |||
**64 | |||
**58 | |||
**0.61 mL in 13 mL and 5.35 mL in 15 mL | |||
* ELISA {{todo|SEE DOSE EFFECT CURVE}} | |||
==Conclusion== | ==Conclusion== | ||
*{{done}} Ow.. | *{{done}} Ow.. | ||
===Related entries=== | |||
====Run 2: Ht31 Dose/Effect Curve & S<sup>0</sup> D9/D12==== | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19|Counting cells 19Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23|Putting to S<sup>0</sup> 23Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Stimulating cells 24Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25|Ending stimulation & Viability 25Feb2010]] | |||
==Same action== | |||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18| ELISA]] | |||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting splitting cells]] | |||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19| Counting splitting cells]] | |||
==Attachment== | |||
<html> | |||
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> | |||
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | |||
</a> | |||
</html> [[Media:HTERT_Donor_9_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D9]] | |||
<br> | |||
<html> | |||
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> | |||
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | |||
</a> | |||
</html> [[Media:HTERT_Donor_12_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D12]] | |||
Revision as of 02:23, 23 March 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
ELISA & Counting splitting cells | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||
Summary
Materials & MethodsMaterialsMethodELISA
Counting and splitting cellsResults
Conclusion
Related entriesRun 2: Ht31 Dose/Effect Curve & S0 D9/D12
Same actionAttachment<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a>
</html> Excel Dose Effect curve D9
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> Excel Dose Effect curve D12
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