User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/12
|Counting cells|| Main project page|
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To ensure proper comparison of results is possible it is advisable to work with comparable amount of cells in different samples. For this it is required to count cells and calculate how many should be added to new wells.
Materials & Methods
Image 120220101: Bürker-Türk counting chamber; Each chamber consists of 4 big blocks, like the square shown here in centre. Each big block consists of 16 small blocks (0.04 m2. The 4 big blocks are separated from the rest by 3 lines. Each row of squares is separated from the other by 2 lines (picture shows 3). Cells are counting within the 16 smaller squares and the spaces in between the rows. However only 2 of the 4 boundary spaces represented by the 3 lines are counted.
Run 1: Ht31/Ht31P D9/D12