IGEM:Imperial/2010/Fast Response module: Difference between revisions

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{{Imperial2010/Header}}
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|+ Table of ideas
|+ Ideas
|-
|-
   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz)
   ! Signalling
|-
|-
   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless
   | [[IGEM:Imperial/2010/Detection_module/2_components_systems | 2 components systems]]
|-
|-
   | Activation (phosphorylation)
   | logic gate
   | Enz-in-pathway
|-
   | Vibrio cholera
|}
 
==Idea 1==
The first idea, that seems to be feasible considering all the components, is the one below:
 
[[Image:OmpR system pic.jpg]]


* short pathways
This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.
* ensure - colourless -> colour
 
|-
 
  | [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha
References:
* not many AA
 
* non-toxic
*The split protease TEV: [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html Monitoring regulated protein-protein interactions using split TEV]
* can work in E.Coli
 
* quantize speed, efficiency
*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641 The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]
  | Protein scaffold: Maddie


|-
*all about EnvZ [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/envz_details.html]
  | 2C DNA binding prot release ||
|}


James- some suggested papers:
*all about OmpR [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/ompr_details.html]
<biblio>
#1 pmid=17117150
#2 pmid=17072307
</biblio>

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Ideas
Signalling
2 components systems
logic gate
Vibrio cholera

Idea 1

The first idea, that seems to be feasible considering all the components, is the one below:

This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.


References:

  • all about EnvZ [1]
  • all about OmpR [2]
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