Swartz:Protocols

   

Research

Papers

People

Contact

Getting settled in the Swartz Lab

Protocols

Cloning

• Swartz Lab Molecular Biology Handbook
• Restriction Enzyme Library

DNA Design

• Primer Design

Gene Synthesis

• PCR
• Gene Synthesis
• Restriction Enzyme Digestion/Ligations
• Quikchange
• Gel Purification
• Column Regeneration

Expression Templates

Plasmids

• OWW Plasmid Miniprep or | Swartz Lab Version
• Plasmid Maxiprep

Organisms

• E.coli Transformation

• Other Cloning Protocols

Cell Free Protein Synthesis

• 1L Extract Prep
• Cell Free Protein Synthesis
• How to do an Autoradiogram
• Western Blotting

Protein Purification

• His-Tag Purification

Assays

• Nanodrop
• Sample prep for MS
• Cytochrome C
• Methyl Viologen
• H2 Production (GC)

Miscellaneous

• Anaerobic Protein Production

Solutions

• Buffer Recipes