Jacobs:Back Door: Difference between revisions
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==Useful Links / Meetings== | ==Useful Links / Meetings== | ||
*[https://suehsaps3.stanford.edu/stanford/ Chemtracker] | *[https://suehsaps3.stanford.edu/stanford/ Chemtracker] | ||
*[http://www.ors.org/ ORS] | *[http://www.ors.org/ ORS] | ||
*[http://asmeconferences.org/ | *[http://www.asmeconferences.org/SBC2012/ ASME SBC] | ||
*[http://www.asbmr.org/ ASBMR] | *[http://www.asbmr.org/ ASBMR] | ||
*[http://www.bmes.org/ BMES] | *[http://www.bmes.org/ BMES] | ||
==Protocols== | ==Protocols== | ||
===Research Protocols=== | ===Research Protocols=== | ||
*[[Jacobs:Protocol Safety|Safety]] | *General | ||
*[[Jacobs:Protocol | **[[Jacobs:Protocol Printers | Printers]] | ||
*[[Jacobs:Protocol | **[[Jacobs:Protocol Safety|Safety]] | ||
*[[Jacobs: | **[[Jacobs:Protocol Autoclave | Autoclave]] | ||
*[[Jacobs:Protocol | **[[Jacobs: Leica MP Microscope Protocol| Leica MP Microscope]] | ||
*[[Jacobs:Protocol | *Cells | ||
*[[Jacobs:Protocol | **Culture | ||
*[[Jacobs:Protocol | ***[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells | Culture of MLO-Y4s]] | ||
*[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]] | ***[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]] | ||
*[[Jacobs:Protocol | ***[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]] | ||
*[[Jacobs: | ***[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Culture of 3T3 Fibroblasts]] | ||
*[[Jacobs:Protocol | ***[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | ||
*[[Jacobs:Protocol | ***[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | ||
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | ***[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]] | ||
*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | **Transfection | ||
*[[Jacobs:Protocol | ***[[Jacobs:Protocol siRNA transfection|siRNA transfection]] | ||
*[[Jacobs:Protocol | ***[[Jacobs:Protocol Neon Transfection | Neon Transfection]] | ||
*[[Jacobs: | ***[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]] | ||
*[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining | **[[Jacobs:Protocol Coating Slides with Fibronectin | Coating with Fibronectin]] | ||
*[[Jacobs: | **[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | ||
*[[Jacobs: | **[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]] | ||
*[[Jacobs: | **[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]] | ||
*[[Jacobs: | *Flow | ||
*[[Jacobs: Protocol | **[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | ||
*[[Jacobs: | **[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | ||
*[[Jacobs: | **[[Jacobs:Confocal Imaging of Flow| Confocal imaging of primary cilia under flow]] | ||
* | |||
*[[Jacobs:Protocol | *Immunocytochemistry | ||
*[[Jacobs:Protocol | **[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | ||
*[[Jacobs:Protocol | **[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]] | ||
*[[Jacobs:Protocol | **[[Jacobs:Protocol Actin Staining|Actin Staining]] | ||
*[[Jacobs:Protocol | **[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]] | ||
*[[Jacobs: | *Assays | ||
*[[ | **[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein Content]] | ||
*[[Jacobs: Protocol | **[[Jacobs:Protocol Real-Time PCR|Real-Time RT qPCR]] | ||
*[[Jacobs: Protocol | **[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]] | ||
**[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase]] | |||
*Gels & blots | |||
**Protein | |||
***[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]] | |||
***[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]] | |||
***[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein SDS-PAGE and Coomassie Staining of Polyacrylamide Gel for Protein]] | |||
**DNA & RNA | |||
***[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]] | |||
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]] | |||
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]] | |||
***[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]] | |||
* Mice | |||
**[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | |||
**[[Jacobs:Genotyping Tailsnips|Genotyping by qPCR]] | |||
**[[Media:Genotyping Tailsnips.doc | Genotyping by agarose gel]] | |||
**[[Jacobs:Protocol Loading Mice|Loading Mice]] | |||
**[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | |||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
*[[Jacobs:Protocol | *[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]] | ||
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]] | *[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]] | ||
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]] | *[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]] | ||
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]] | *[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]] | ||
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]] | *[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]] | ||
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]] | |||
*[[Jacobs:Protocol | |||
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]] | *[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]] | ||
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]] | *[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]] | ||
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]] | *[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]] | ||
==Miscellaneous== | ==Miscellaneous== | ||
* [[Photos:Jacobs photos]] | * [[Photos:Jacobs photos]] | ||
* [[Jacobs:Library|Library]] | |||
* [http://www.stanford.edu/group/cmbl/movies.htm Movies] | * [http://www.stanford.edu/group/cmbl/movies.htm Movies] | ||
</div> |
Latest revision as of 12:49, 2 December 2014
Useful Links / Meetings
Protocols
Research Protocols
- General
- Cells
- Flow
- Immunocytochemistry
- Assays
- Gels & blots
- Protein
- DNA & RNA
- Mice
Teaching Protocols
- Pipetting Exercise
- Adipocyte Differentiation
- Oil Red O Staining
- RNA Agarose Gel
- Collagen Gel Contraction
- Collagen Gel Contraction 2
- Total Protein Isolation using RIPA buffer
- Total Protein Isolation using RIPA buffer 2
- RNA Sample Loading Buffer