User:Jessica Karen Wong/Progress: Difference between revisions
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*F2620 has Mfe/BB prefix tails | *F2620 has Mfe/BB prefix tails | ||
* | *Ligated into 3K3 and did correct transformation into DB3.1 | ||
*Waiting on seq verification | *Waiting on seq verification | ||
*E0240 has been PCR'ed with tails, looks the right size | *E0240 has been PCR'ed with tails, looks the right size | ||
* | *Digested E0240 S/N to ligate into F2620 | ||
*Will ligate into the correctly seq'ed F2620 colony | |||
==E0240== | ==E0240== | ||
| Line 22: | Line 23: | ||
*Has a 1bp mutation around 700 | *Has a 1bp mutation around 700 | ||
*Did mutagenesis and got a good number of colonies | *Did mutagenesis and got a good number of colonies | ||
* | *EX version has been confirmed correct - 8/9 | ||
*Made overnights to seq more ES colonies | |||
==I2057== | ==I2057== | ||
| Line 34: | Line 36: | ||
*Inserted a BB site b/t plasmid and RBS | *Inserted a BB site b/t plasmid and RBS | ||
*Created both E/X and E/S versions | *Created both E/X and E/S versions | ||
*E/S sealed w/ Not1 is seq verified | *E/S sealed w/ Not1 is seq verified - 8/6 | ||
*E/X sealed w/ Not1 is seq verified | *E/X sealed w/ Not1 is seq verified - 8/6 and 8/10 | ||
Ligated J116, J100, and R0040 into EX version from PCR - | Ligated J116, J100, and R0040 into EX version from PCR | ||
*R0040 in both EX and ES versions is perfect - 8/9 | |||
==I2055== | ==I2055== | ||
| Line 43: | Line 46: | ||
<BBpart> I2055</BBpart> | <BBpart> I2055</BBpart> | ||
* | *Built and scarred - 7/31 and 8/2 | ||
*Inserted a BB site b/t Promoter and GFP | *Inserted a BB site b/t Promoter and GFP | ||
*Made both E/X and S/X forms | *Made both E/X and S/X forms | ||
*Sealed both EX and SX with Not1 - waiting on seq | *Sealed both EX and SX with Not1 - waiting on seq | ||
*Inserted B0034 into both EX and SX versions of PCR, | *Inserted B0034 and B0032 into both EX and SX versions of PCR, also B0030, B0031 into SX PCR | ||
*I2055-SX-B0031 is seq verified 8/9 | |||
==I2056== | ==I2056== | ||
Revision as of 19:52, 13 August 2007
T9002
Output Measurement Kit (for inverters, terminators, etc) Using GFP
<BBpart> T9002</BBpart>
- F2620 has Mfe/BB prefix tails
- Ligated into 3K3 and did correct transformation into DB3.1
- Waiting on seq verification
- E0240 has been PCR'ed with tails, looks the right size
- Digested E0240 S/N to ligate into F2620
- Will ligate into the correctly seq'ed F2620 colony
E0240
Promoter Tester with GFP
<BBpart> E0240</BBpart>
- Part is built
- PCRed with Mfe1 and Spe1 tails
- Successfully ligated into 3K3 - Sequence verifies that it's scarred
- Inserted a BB site between plasmid and RBS
- Made both E/S and E/X versions
- Has a 1bp mutation around 700
- Did mutagenesis and got a good number of colonies
- EX version has been confirmed correct - 8/9
- Made overnights to seq more ES colonies
I2057
Promoter tester with RFP
<BBpart> I2057</BBpart>
- Part is built (sequence verified)
- PCR-ed with Mfe1 and Nsi1
- Ligated in 3K3 (seq verified scarred)
- Inserted a BB site b/t plasmid and RBS
- Created both E/X and E/S versions
- E/S sealed w/ Not1 is seq verified - 8/6
- E/X sealed w/ Not1 is seq verified - 8/6 and 8/10
Ligated J116, J100, and R0040 into EX version from PCR
- R0040 in both EX and ES versions is perfect - 8/9
I2055
RBS tester with GFP
<BBpart> I2055</BBpart>
- Built and scarred - 7/31 and 8/2
- Inserted a BB site b/t Promoter and GFP
- Made both E/X and S/X forms
- Sealed both EX and SX with Not1 - waiting on seq
- Inserted B0034 and B0032 into both EX and SX versions of PCR, also B0030, B0031 into SX PCR
- I2055-SX-B0031 is seq verified 8/9
I2056
RBS tester with RFP
<BBpart> I2056</BBpart>
- Formerly called blue
- Part is most likely built and scarred
- Waiting on redo of seq to make sure front is scarred
- Inserted a BB site b/t promoter and RFP
- Created both E/X and S/X
- Attempting to seal both varieties and has same RBS's as I55
