Talk:20.109(F07):Start-up genome engineering: Difference between revisions
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| Line 47: | Line 47: | ||
| TRpink_top | | TRpink_top | ||
| NO | | NO | ||
| | | 5’ GAT CTC GAC GGC GCC 3’ | ||
| | | | ||
| | | Sal I | ||
|- | |- | ||
| TRpink_btm | | TRpink_btm | ||
| NO | | NO | ||
| | | 5’ G ATC GGC GCC GTC GA 3’ | ||
| | | | ||
|Sfo I | |||
|- | |- | ||
| TRyellow_top | | TRyellow_top | ||
Revision as of 20:31, 11 September 2007
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
|---|---|---|---|---|---|
| TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
| TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
| M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
| M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
|---|---|---|---|---|
| TRpink_top | NO | 5’ GAT CTC GAC GGC GCC 3’ | Sal I | |
| TRpink_btm | NO | 5’ G ATC GGC GCC GTC GA 3’ | Sfo I | |
| TRyellow_top | NO | |||
| TRyellow_btm | NO | |||
| TRblue_top | NO | |||
| TRblue_btm | NO | |||
| TRpurple_top | NO | |||
| TRpurple_btm | NO | |||
| TRred_top | NO | |||
| TRred_btm | NO | |||
| TRgreen_top | NO | |||
| TRgreen_btm | NO |
W/F lab
| oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
|---|---|---|---|---|
| WFpink_top | NO | |||
| WFpink_btm | NO | |||
| WFyellow_top | NO | |||
| WFyellow_btm | NO | |||
| WFblue_top | NO | |||
| WFblue_btm | NO | |||
| WFpurple_top | NO | |||
| WFpurple_btm | NO | |||
| WFred_top | NO | |||
| WFred_btm | NO | |||
| WFgreen_top | NO | |||
| WFgreen_btm | NO |
