Prince:UPLC: Difference between revisions
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New page: == Flow Rates == Table 2-3. Effective flow rate range SilicaTip emitters (from Finnigan Nanospray Ion Source Operator’s Manual 97055-97025 Revision A, October 2004) {| border="1" ! Emitt... |
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Revision as of 17:15, 2 June 2010
Flow Rates
Table 2-3. Effective flow rate range SilicaTip emitters (from Finnigan Nanospray Ion Source Operator’s Manual 97055-97025 Revision A, October 2004)
| Emitter ID (um) | Flow rate (nL/min) |
|---|---|
| 5 | 20 to 100 |
| 8 | 100 to 300 |
| 10 | 100 to 400 |
| 15 | 150 to 400 |
| 30 | 300 to 1000 |
Table 9-1. LC/NSI/MS Parameters for Finnigan LTQ or LCQ Series mass spectrometers (Finnigan™
Nanospray Ion Source Operator’s Manual 97055-97025 Revision A October 2004)
| Column Size | Flow Rate | Tip ID | Emitter Position | Capillary Temperature |
|---|---|---|---|---|
| 50 μm | <100 to 300 nL/min | 5 to 15 μm | approx. 1 mm | 100 to 180 °C |
| 75 μm | 150 to 800 nL/min | 10 to 20 μm | 1 to 2 mm | 130 to 200 °C |
| 180 μm | 0.3 to 1.0 μL/min | 20 to 30 μm | 2 to 3 mm | 150 to 250 °C |
| 320 μm | 1 to 5 μL/min | 30 to 75 μm | 3 to 5 mm | 150 to 250 °C |
This is from the Thermo Chromatography Catalogue 2006-2007 (pg. 177).
| ColumnID (mm) | Flow Rate Range (uL/min) | Optimum Flow Rate (ul/min) | Recommended Injection Vol. (ul) | API Source |
|---|---|---|---|---|
| 0.32 | 4 - 10 | 5 | 0.15 | Micro-ESI |
| 0.18 | 1 - 3 | 2 | 0.05 | Micro-ESI |
| 0.1 | 0.4 - 1 | 0.5 | 0.015 | Nanospray |
| 0.075 | 0.2 - 0.5 | 0.3 | 0.01 | Nanospray |
