IGEM:Harvard/2006/Cyanobacteria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 68: | Line 68: | ||
*[[Media:Cyano_week4.ppt |Week 4 progress update]] | *[[Media:Cyano_week4.ppt |Week 4 progress update]] | ||
*[[Media:Cyanobacteria_Presentation_Week_5.ppt |Week 5 progress update, upd. 10:10 7/17]] | *[[Media:Cyanobacteria_Presentation_Week_5.ppt |Week 5 progress update, upd. 10:10 7/17]] | ||
*[[Media:Cyanobacteria_Presentation_Week_6.ppt |Week 6 progress update, upd. 10: | *[[Media:Cyanobacteria_Presentation_Week_6.ppt |Week 6 progress update, upd. 10:02 7/24 HH]] | ||
==Team Members== | ==Team Members== |
Revision as of 07:01, 24 July 2006
<html><style type='text/css'> .tabs {
font-size:80%; font-weight:none; width: 100%; color: #FFFFFF; background:#FFFFFF url("/images/5/54/DarkgreenTab-bg.gif") repeat-x bottom;
}
.tabs li {
background:url("/images/3/36/DarkgeenTab-left.gif") no-repeat left top;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top; color:#FFFFFF; padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#CCFF00; background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:#66FF00;
}
</style></html>
Synthesis
Agenda
See image at right for our long-term project outline.
Monday 6/26
- Figure out optimal growing conditions (complete)
- Regrow cyanobacteria colonies (complete)
- Draft email questions for Professor Golden (complete)
Tuesday 6/27
- PCR crash course (complete)
- Picnic! (complete)
- Check first batch of colonies again (complete)
- Complete more research in preparation for Professor Golden (complete)
- Order primers (complete)
Wednesday 6/28
- Research transformation protocols (mostly complete)
- Start creating presentation for Friday
- Talk to Professor Golden (complete)
- Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
- Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)
Thursday 6/29
- Research Sigma factors (on hold)
- Design and order our first set of primers (for entire KaiABC sequence) (complete)
- Make presentation for Friday (complete)
Friday 6/30
- Present before Fourth of July weekend (cancelled)
Wednesday 7/5
- Research plasmids to ask Prof. Golden (moved from Thursday)
- Also, don't forget to ask her about CO2 and aerating our cultures
- Find out what equipment we would need for measuring bioluminescence
Thursday 7/6
- Make a model of the cyanobacteria
- We missed ice cream at Maxwell-Dworkin
Friday 7/7
- Make a model of the cyanobacteria (complete)
- We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
- PCR PCC7942 (complete-- turned out negative)
- Plate PCC7942 (complete)
- Grow PCC7942 in liquid culture, both with thiosulfate and without (complete)
- TGIF in Bauer Center (complete)
Monday 7/10
- Make frozen stocks of our new grown PCC 7942 (complete)
- Research PCR protocols for cyanobacteria (maybe?) (complete)
- PCR (complete)
- Research DNA synthesis companies (complete)
- Design sequence to be synthesized (incomplete)
- Research homologous recombination design (incomplete)
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
- Week 6 progress update, upd. 10:02 7/24 HH
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
List of abbreviations:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
23 May 2024
|
08:32 | Park Lab :Publications 2 changes history +411 [Jeewoong Lee (2×)] | |||
|
08:32 (cur | prev) +1 Jeewoong Lee talk contribs (→Publications (*†equal contribution)) | ||||
|
08:31 (cur | prev) +410 Jeewoong Lee talk contribs (→Publications (*†equal contribution)) |
06:49 | Xu lab:Publications diffhist +105 Peisheng Xu talk contribs |
22 May 2024
08:51 | Beauchamp:Publications diffhist 0 Michael S Beauchamp talk contribs |
07:26 | Multilayer Paper Microfluidics - Madyson Redder diffhist +51 Sarah L. Perry talk contribs (→Overview) |
|
07:13 | Capillary Electrophoresis - Andrew Maloney, Tim Towner, Camryn Payne 7 changes history −347 [Sarah L. Perry (7×)] | |||
|
07:13 (cur | prev) +112 Sarah L. Perry talk contribs | ||||
|
07:07 (cur | prev) −3 Sarah L. Perry talk contribs (→Background) | ||||
|
07:07 (cur | prev) 0 Sarah L. Perry talk contribs (→Background) | ||||
|
07:06 (cur | prev) +145 Sarah L. Perry talk contribs (→Background) | ||||
|
07:04 (cur | prev) −314 Sarah L. Perry talk contribs | ||||
|
06:42 (cur | prev) −198 Sarah L. Perry talk contribs (→References) | ||||
|
06:38 (cur | prev) −89 Sarah L. Perry talk contribs (→Background) |
21 May 2024
19:10 | CHEM-ENG590E:Wiki Textbook diffhist −37 Sarah L. Perry talk contribs (→Chapter 9 - Microfluidics and Cell Culture) |
19:09 | Move log Sarah L. Perry talk contribs moved page Organ/Tumor/Body-on-a-Chip - Organ on a Chip - Michele Caggioni to Organ/Tumor/Body-on-a-Chip (More appropriate title) |