Altman:Protocols/Preparing Reagents/Making assay buffer: Difference between revisions
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*100x DTT stock: 100 mM dithiothreitol | *100x DTT stock: 100 mM dithiothreitol | ||
*10x BSA stock: 10 mg/mL bovine serum albumin | *10x BSA stock: 10 mg/mL bovine serum albumin | ||
*10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM | *10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl_2, | ||
Revision as of 01:41, 11 November 2012
Department of Physics, Willamette University
Making Assay Buffer
Prepare the following beforehand:
- 100x DTT stock: 100 mM dithiothreitol
- 10x BSA stock: 10 mg/mL bovine serum albumin
- 10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl_2,
| 1 mL Assay Buffer | 1 mL Assay Buffer + BSA | |
|---|---|---|
| 10x AB stock | 100 uL | 100 uL |
| 100x DTT stock | 10 uL | 10 uL |
| 10x BSA stock | -- | 100 uL |
| ddwater | 890 uL | 790 uL |
