Altman:Protocols/Preparing Reagents/Making assay buffer

Department of Physics, Willamette University

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Making Assay Buffer

Prepare the following beforehand:

• 100x DTT stock: 100 mM dithiothreitol
• 10x BSA stock: 10 mg/mL bovine serum albumin
• 10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl₂

	1 mL Assay Buffer (AB)	1 mL Assay Buffer + BSA (ABSA)
10x AB stock	100 uL	100 uL
100x DTT stock	10 uL	10 uL
10x BSA stock	--	100 uL
ddwater	890 uL	790 uL