Altman:Protocols/Preparing Reagents/Making assay buffer

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Department of Physics, Willamette University

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Making Assay Buffer

Prepare the following beforehand:

  • 100x DTT stock: 100 mM dithiothreitol
  • 10x BSA stock: 10 mg/mL bovine serum albumin
  • 10x AB stock: 250 mM KCl, 250 mM Imidazole-Cl (pH 7.4), 10 mM EGTA, 40 mM MgCl2


1 mL Assay Buffer

(AB)

1 mL Assay Buffer + BSA

(ABSA)

10x AB stock 100 uL 100 uL
100x DTT stock 10 uL 10 uL
10x BSA stock -- 100 uL
ddwater 890 uL 790 uL
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