Wittrup:Protocols
From OpenWetWare
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Plasmid minipreps, restriction digestion
Wittrup: Gel electrophoresis and band purification
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Wittrup:Protein conjugation with fluorophores
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Cell culture
Wittrup: Cell culture protocols
