Wittrup: Plasmid rescue from yeast

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Use zymoprep I kit [1] or zymoprep II kit [2] from ZymoResearch.


Zymoprep I: Modifications to manufacturer's protocol -- Resuspend pellet in 17 uL TE and use 1-2 uL for XLI Blue transformation.


Zymoprep II: Modifications to manufacturer's protocol -- Spin down supernatant in fresh tubes a second time before applying to column. -- Elute column in 20 uL TE, and then clean this product on a Qiagen column.

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