Griffitts:Bacterial Strains
From OpenWetWare
| Strain # | Parental strain | Plasmid | Marker | Notes |
|---|---|---|---|---|
| B001 | DH5α | pRK600 | CmR | pRK600 from MT616; demonstrated to be a functional helper strain |
| B024 | RM1021 | None | None | ΔSmb21188 (acyl-T) |
| B025 | RM1021 | None | None | ΔSmb21189 and ΔSmb21190 (glycosyltransferases) |
| B026 | RM1021 | None | None | ΔSmb21191 (msbA2) |
| B042 | RM1021 | pTB93F | None | gfp+ |
| B043 | DH5α | pXLGD4 | TcR | hemA::lacZ in pRK290 |
| B044 | DH5α | pTB93F | SpR | Ptrp::gfp S65T in pMB393 |
| B060 | M. loti | None | FmR | MAFF303099; grows on TY-fosfomycin100 |
| B061 | XL1-Blue | pMAL-C2G | None | |
| B062 | XL1-Blue | pGEX-5X-1 | None | |
| B063 | DH5α | pGEX-4T-1 | None | |
| B068 | XL1-Blue | pDG71 | TcR, KmR | plasmid provides Ptrp:GFP (mut3) on pHC41, highly stable in planta |
| B078 | MR1021 | pRF771 | None | control strain |
| B079 | MG1655 | None | None | Wild-type E. coli |
| B081 | MC4100 | None | SmR | F- araD139 Δ(argf-lac)U169* rpsL150 relA1 flb5301 fruA25 deoC1 ptsF25 e14- |
| B082 | BW25139 | None | None | pir+ recA+ |
| B083 | BW25141 | None | None | pir+ recA- (use this one for cloning R6K plasmids) |
| B084 | BW2141 | pGP704 | ApR | pGP704 is pir-dependent |
| B087 | S. fredii | None | None | USDA257 |
| B088 | S. fredii | None | None | NGR234 |
| B089 | A. tumefaciens | None | None | LBA4404 |
| B090 | M. loti | None | None | NZP2037 |
| B091 | R. tropici | None | None | BR816 |
| B093 | B. japonicum | None | None | USDA110 |
| B094 | MG1655 | None | SmR | transduced with lysate from MC4100 |
| B095 | DH5α | None | None | |
| B096 | DH5α | pRF771 | None | |
| B097 | KS1000 | None | None | from NEB |
| B098 | TB1 | None | None | from NEB |
| B099 | DH5α | pJQ200SK | None | May be HB101 instead of DH5α |
| B100 | RM1021 | None | None | |
| B103 | RM1021 | None | None | expR101 (expR+) |
| B119 | RM1021 | None | None | ΔfeuP |
| B122 | RM1021 | None | None | ΔfeuQ |
| B125 | RM1021 | None | None | trsA mutant, trsA::Tn5-110 |
| B146 | RM1021 | None | None | hisH- (interrupted by Tn5-110) |
| B147 | RM1021 | None | None | aroF- (interrupted by Tn5-110) |
| B148 | RM1021 | None | None | carA- (interrupted by Tn5-110) |
| B149 | RM1021 | None | None | ndvA- (interrupted by Tn5-110) |
| B150 | RM1021 | None | None | fixB- (interrupted by Tn5-110) |
| B151 | RM1021 | None | None | dme- (interrupted by Tn5-110) |
| B152 | RM1021 | None | None | fixI- (interrupted by Tn5-110) |
| B153 | RM1021 | None | None | exoB- (interrupted by Tn5-110) |
| B154 | RM1021 | None | None | nifK- (interrupted by Tn5-110) |
| B155 | RM1021 | None | None | thrB- (interrupted by Tn5-110) |
| B156 | RM1021 | None | None | tkt2- (interrupted by Tn5-110) |
| B157 | RM1021 | None | None | ccmB- (interrupted by Tn5-110) |
| B158 | RM1021 | None | None | exoF- (interrupted by Tn5-110) |
| B159 | RM1021 | None | None | pckA- (interrupted by Tn5-110) |
| B160 | RM1021 | None | None | carB- (interrupted by Tn5-110) |
| B161 | RM1021 | None | None | relA- (interrupted by Tn5-110) |
| B162 | RM1021 | None | None | bacA- (interrupted by Tn5-110) |
| B163 | RM1021 | None | None | eno- (interrupted by Tn5-110) |
| B164 | RM1021 | None | None | cycJ- (interrupted by Tn5-110) |
| B165 | RM1021 | None | None | unknown gene essential for symbiosis interrupted by Tn5-110 |
| B166 | RM1021 | None | None | dctA- (interrupted by Tn5-110) |
| B167 | RM1021 | None | None | phaA1- (interrupted by Tn5-110) |
| B168 | RM1021 | None | None | ilvD2- (interrupted by Tn5-110) |
| B169 | RM1021 | None | None | nodH- (interrupted by Tn5-110) |
| B170 | RM1021 | None | None | leuD- (interrupted by Tn5-110) |
| B171 | RM1021 | None | None | exoY- (interrupted by Tn5-110) |
| B172 | RM1021 | None | None | fixG- (interrupted by Tn5-110) |
| B173 | RM1021 | None | None | leuB- (interrupted by Tn5-110) |
| B174 | RM1021 | None | None | dctB- (interrupted by Tn5-110) |
| B175 | RM1021 | None | None | phoC- (interrupted by Tn5-110) |
| B176 | RM1021 | None | None | phoE- (interrupted by Tn5-110) |
| B177 | RM1021 | None | None | gltD- (interrupted by Tn5-110) |
| B178 | RM1021 | None | None | ccmC- (interrupted by Tn5-110) |
| B179 | RM1021 | None | None | purF- (interrupted by Tn5-110) |
| B180 | RM1021 | None | None | unknown gene essential for symbiosis interrupted by Tn5-110 |
| B181 | RM1021 | None | None | hisF- (interrupted by Tn5-110) |
| B181 | RM1021 | None | None | hisF- (interrupted by Tn5-110) |
| B181 | RM1021 | None | None | hisF- (interrupted by Tn5-110) |
| B182 | RM1021 | None | None | cycH- (interrupted by Tn5-110) |
| B183 | RM1021 | None | None | leuC- (interrupted by Tn5-110) |
| B184 | RM1021 | None | None | leuA- (interrupted by Tn5-110) |
| B185 | RM1021 | None | None | nifB- (interrupted by Tn5-110) |
| B186 | RM1021 | None | None | fixX- (interrupted by Tn5-110) |
| B187 | RM1021 | None | None | feuQ- (interrupted by Tn5-110) |
| B188 | RM1021 | None | None | feuP- (interrupted by Tn5-110) |
| B189 | RM1021 | None | None | fixL- (interrupted by Tn5-110) |
| B190 | RM1021 | None | None | trpE- (interrupted by Tn5-110) |
| B191 | RM1021 | None | None | purK- (interrupted by Tn5-110) |
| B192 | RM1021 | None | None | purL- (interrupted by Tn5-110) |
| B193 | RM1021 | None | None | purC- (interrupted by Tn5-110) |
| B194 | RM1021 | None | None | phoD- (interrupted by Tn5-110) |
| B195 | RM1021 | None | None | ilvI- (interrupted by Tn5-110) |
| B196 | RM1021 | None | None | exoP- (interrupted by Tn5-110) |
| B197 | RM1021 | None | None | nodB- (interrupted by Tn5-110) |
| B198 | RM1021 | None | None | cycK- (interrupted by Tn5-110) |
| B199 | RM1021 | None | None | lpsB- (interrupted by Tn5-110) |
| B200 | RM1021 | None | None | metF- (interrupted by Tn5-110) |
| B201 | RM1021 | None | None | ccmG- (interrupted by Tn5-110) |
| B202 | RM1021 | None | None | nifN- (interrupted by Tn5-110) |
| B203 | RM1021 | None | None | rpoN- (interrupted by Tn5-110) |
| B204 | RM1021 | None | None | ccsA- (interrupted by Tn5-110) |
| B205 | RM1021 | None | None | exoD- (interrupted by Tn5-110) |
| B206 | RM1021 | None | None | exoH- (interrupted by Tn5-110) |
| B207 | RM1021 | None | None | thiG- (interrupted by Tn5-110) |
| B208 | RM1021 | None | None | phaG1- (interrupted by Tn5-110) |
| B209 | RM1021 | None | None | nifE- (interrupted by Tn5-110) |
| B210 | RM1021 | None | None | ndvB- (interrupted by Tn5-110) |
| B211 | RM1021 | None | None | thiF- (interrupted by Tn5-110) |
| B212 | RM1021 | None | None | msbA2- (interrupted by Tn5-110) |
| B213 | RM1021 | None | None | purM- (interrupted by Tn5-110) |
| B214 | RM1021 | None | None | fixC- (interrupted by Tn5-110) |
| B215 | RM1021 | None | None | exoL- (interrupted by Tn5-110) |
| B216 | RM1021 | None | None | cycL- (interrupted by Tn5-110) |
| B244 | DH5α | pMAL-C2G | None | Plasmid from E. Wilson |
| B245 | RM1021 | pRF771 | SmR, TcR | |
| B250 | RM1021 | pJG230 | None | ΔgutR, reference deletion strain |
| B304 | RM1021 | pJG225 | NmR | |
| B306 | B119 | pJG225 | NmR | |
| B308 | B122 | pJG225 | NmR | |
| B349 | RM1021 | pJG271 | SmR, TcR | Tavr + lacZ; canonical strain for β-gal assays |
| B355 | RM1021 | pJG280 | SmR, TcR | Me1 promoter + lacZ; canonical strain for β-gal assays |
| B372 | BW25113 | pKD46 | None | λ red vector |
| B378 | DH5α | pMB393, pBBR | SpR | |
| B379 | RM1021 | pJG286 | TcR | |
| B380 | B188 | pJG286 | TcR | |
| B381 | MG1655 | None | None | lac white mutant |
| B382 | MG1655 | None | None | lac blue mutant |
| B385 | DH5α | pTE3 | TcR | |
| B386 | DH5α | pE65 | TcR | |
| B387 | DH5α | pMB89 | TcR | |
| B389 | MC4100 | pKD46 | ApR | For making λ red knockouts; grow at 30°C |
| B390 | MC4100 | pJG288 | KmR | For transposon mutagenesis |
| B392 | MG1655 | None | SmR | resistance transduced from MC4100 |
| B393 | DH5α | None | SmR | resistance arose spontaneously |
| B453 | V02675 | None | SmR, NmR | fixJ2.3::Tn5 |
| B454 | RM7095 | None | SmR, NmR | exoR95::Tn5 |
| B455 | RM7096 | None | SmR, NmR | exoS96::Tn5 |
| B456 | MB801 | None | SmR, NmR | exoX::Tn5-100 |
| B457 | MB817 | None | SmR, NmR | intergenic Tn5-100 next to syrM |
| B458 | MB846 | None | SmR, NmR | intergenic Tn5-100 next to exoY |
| B459 | MB859 | None | SmR, NmR | intergenic Tn5-100 between expD1 and expG |
