Griffitts:Bacterial Strains

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Griffitts Lab Bacterial Strains
Strain # Parental strain Plasmid Marker Notes
B001 DH5α pRK600 CmR pRK600 from MT616; demonstrated to be a functional helper strain
B024 RM1021 None None ΔSmb21188 (acyl-T)
B025 RM1021 None None ΔSmb21189 and ΔSmb21190 (glycosyltransferases)
B026 RM1021 None None ΔSmb21191 (msbA2)
B042 RM1021 pTB93F None gfp+
B043 DH5α pXLGD4 TcR hemA::lacZ in pRK290
B044 DH5α pTB93F SpR Ptrp::gfp S65T in pMB393
B060 MAFF303099 None FmR M. loti; grows on TY-fosfomycin100
B061 XL1-Blue pMAL-C2G None
B062 XL1-Blue pGEX-5X-1 None
B063 DH5α pGEX-4T-1 None
B068 XL1-Blue pDG71 TcR, KmR plasmid provides Ptrp:GFP (mut3) on pHC41, highly stable in planta
B078 Rm1021 pRF771 SmR, TcR control strain
B079 MG1655 None None Wild-type E. coli
B081 MC4100 None SmR F araD139 Δ(argf–lac)U169* rpsL150 relA1 flb5301 fruA25 deoC1 ptsF25 e14
B082 BW25139 None None pir+ recA+
B083 BW25141 None None pir+ recA (use this one for cloning R6K plasmids)
B084 BW2141 pGP704 ApR pGP704 is pir-dependent
B087 USDA 257 None None S. fredii
B088 NGR234 None None S. fredii
B089 LBA4404 None None A. tumefaciens
B090 NZP2037 None None M. loti
B091 BR816 None None R. tropici
B093 USDA 110 None None B. japonicum
B094 MG1655 None SmR transduced with lysate from MC4100
B095 DH5α None None
B096 DH5α pRF771 TcR
B097 KS1000 None None from NEB
B098 TB1 None None from NEB
B099 DH5α pJQ200SK None May be HB101 instead of DH5α
B100 RM1021 None None
B103 RM1021 None None expR101 (expR+)
B119 RM1021 None None ΔfeuP
B122 RM1021 None None ΔfeuQ
B125 RM1021 None None trsA mutant, trsA::Tn5-110
B146 RM1021 None None hisH (interrupted by Tn5-110)
B147 RM1021 None None aroF (interrupted by Tn5-110)
B148 RM1021 None None carA (interrupted by Tn5-110)
B149 RM1021 None None ndvA (interrupted by Tn5-110)
B150 RM1021 None None fixB (interrupted by Tn5-110)
B151 RM1021 None None dme (interrupted by Tn5-110)
B152 RM1021 None None fixI (interrupted by Tn5-110)
B153 RM1021 None None exoB (interrupted by Tn5-110)
B154 RM1021 None None nifK (interrupted by Tn5-110)
B155 RM1021 None None thrB (interrupted by Tn5-110)
B156 RM1021 None None tkt2 (interrupted by Tn5-110)
B157 RM1021 None None ccmB (interrupted by Tn5-110)
B158 RM1021 None None exoF (interrupted by Tn5-110)
B159 RM1021 None None pckA (interrupted by Tn5-110)
B160 RM1021 None None carB (interrupted by Tn5-110)
B161 RM1021 None None relA (interrupted by Tn5-110)
B162 RM1021 None None bacA (interrupted by Tn5-110)
B163 RM1021 None None eno (interrupted by Tn5-110)
B164 RM1021 None None cycJ (interrupted by Tn5-110)
B165 RM1021 None None unknown gene essential for symbiosis interrupted by Tn5-110
B166 RM1021 None None dctA (interrupted by Tn5-110)
B167 RM1021 None None phaA1 (interrupted by Tn5-110)
B168 RM1021 None None ilvD2 (interrupted by Tn5-110)
B169 RM1021 None None nodH (interrupted by Tn5-110)
B170 RM1021 None None leuD (interrupted by Tn5-110)
B171 RM1021 None None exoY (interrupted by Tn5-110)
B172 RM1021 None None fixG (interrupted by Tn5-110)
B173 RM1021 None None leuB (interrupted by Tn5-110)
B174 RM1021 None None dctB (interrupted by Tn5-110)
B175 RM1021 None None phoC (interrupted by Tn5-110)
B176 RM1021 None None phoE (interrupted by Tn5-110)
B177 RM1021 None None gltD (interrupted by Tn5-110)
B178 RM1021 None None ccmC (interrupted by Tn5-110)
B179 RM1021 None None purF (interrupted by Tn5-110)
B180 RM1021 None None unknown gene essential for symbiosis interrupted by Tn5-110
B181 RM1021 None None hisF (interrupted by Tn5-110)
B182 RM1021 None None cycH (interrupted by Tn5-110)
B183 RM1021 None None leuC (interrupted by Tn5-110)
B184 RM1021 None None leuA (interrupted by Tn5-110)
B185 RM1021 None None nifB (interrupted by Tn5-110)
B186 RM1021 None None fixX (interrupted by Tn5-110)
B187 RM1021 None None feuQ (interrupted by Tn5-110)
B188 RM1021 None None feuP (interrupted by Tn5-110)
B189 RM1021 None None fixL (interrupted by Tn5-110)
B190 RM1021 None None trpE (interrupted by Tn5-110)
B191 RM1021 None None purK (interrupted by Tn5-110)
B192 RM1021 None None purL (interrupted by Tn5-110)
B193 RM1021 None None purC (interrupted by Tn5-110)
B194 RM1021 None None phoD (interrupted by Tn5-110)
B195 RM1021 None None ilvI (interrupted by Tn5-110)
B196 RM1021 None None exoP (interrupted by Tn5-110)
B197 RM1021 None None nodB (interrupted by Tn5-110)
B198 RM1021 None None cycK (interrupted by Tn5-110)
B199 RM1021 None None lpsB (interrupted by Tn5-110)
B200 RM1021 None None metF (interrupted by Tn5-110)
B201 RM1021 None None ccmG (interrupted by Tn5-110)
B202 RM1021 None None nifN (interrupted by Tn5-110)
B203 RM1021 None None rpoN (interrupted by Tn5-110)
B204 RM1021 None None ccsA (interrupted by Tn5-110)
B205 RM1021 None None exoD (interrupted by Tn5-110)
B206 RM1021 None None exoH (interrupted by Tn5-110)
B207 RM1021 None None thiG (interrupted by Tn5-110)
B208 RM1021 None None phaG1 (interrupted by Tn5-110)
B209 RM1021 None None nifE (interrupted by Tn5-110)
B210 RM1021 None None ndvB (interrupted by Tn5-110)
B211 RM1021 None None thiF (interrupted by Tn5-110)
B212 RM1021 None None msbA2 (interrupted by Tn5-110)
B213 RM1021 None None purM (interrupted by Tn5-110)
B214 RM1021 None None fixC (interrupted by Tn5-110)
B215 RM1021 None None exoL (interrupted by Tn5-110)
B216 RM1021 None None cycL (interrupted by Tn5-110)
B244 DH5α pMAL-C2G None Plasmid from E. Wilson
B245 RM1021 pRF771 SmR, TcR
B250 RM1021 pJG230 None ΔgutR, reference deletion strain
B262 USDA 1664* None None S. medicae, good promiscuous nodulator; doesn't grow on LB—use TY
B263 USDA 1652* None None S. medicae, good promiscuous nodulator; doesn't grow on LB—use TY
B304 RM1021 pJG225 NmR
B306 B119 pJG225 NmR
B308 B122 pJG225 NmR
B349 RM1021 pJG271 SmR, TcR Tavr + lacZ; canonical strain for β-gal assays
B355 RM1021 pJG280 SmR, TcR Me1 promoter + lacZ; canonical strain for β-gal assays
B372 BW25113 pKD46 None λ red vector
B378 DH5α pMB393, pBBR SpR
B379 RM1021 pJG286 TcR
B380 B188 pJG286 TcR
B381 MG1655 None None lac white mutant
B382 MG1655 None None lac blue mutant
B385 DH5α pTE3 TcR
B386 DH5α pE65 TcR
B387 DH5α pMB89 TcR
B389 MC4100 pKD46 ApR For making λ red knockouts; grow at 30°C
B390 MC4100 pJG288 KmR For transposon mutagenesis
B392 MG1655 None SmR resistance transduced from MC4100
B393 DH5α None SmR resistance arose spontaneously
B453 V02675 None SmR, NmR fixJ2.3::Tn5
B454 RM7095 None SmR, NmR exoR95::Tn5
B455 RM7096 None SmR, NmR exoS96::Tn5
B456 MB801 None SmR, NmR exoX::Tn5-110
B457 MB817 None SmR, NmR intergenic Tn5-110 next to syrM
B458 MB846 None SmR, NmR intergenic Tn5-110 next to exoY
B459 MB859 None SmR, NmR intergenic Tn5-110 between expD1 and expG

*More info can be obtained for the USDA Sinorhizobium meliloti accessions here.

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