IGEM:Harvard/2010/Brainstorming/food

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

protocols

• Book: Agrobacterium Protocols

RNAi

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Markers and Kill Switches

Dual selection system (aka killswitch) ideas

• use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  
• cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  
• cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
  

Downstream effectors

allergens

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1. Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci. 2008 Jun;13(6):257-60. DOI:10.1016/j.tplants.2008.04.004 |

flavors

• Growth dependent banana odor generator, MIT iGEM 2006

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