IGEM:Berkeley/2010

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Berkeley iGEM 2010

Project Name

Project Summary

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Undergrads Amy Kristofferson Conor McClune Tahoura Daniela Christoph

Postdocs

Insert names here

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The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Procedure Overview (Plasmid DNA to Sequencing)
Competent cell production
Plasmid Transformation
Digestion
Ligation
Sequencing
Overlap extension of oligos
PCR prep
Conjugation
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts
Knockouts by Electroporation of pOX38 x pKD46

Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook

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Addressable Cell-to-Cell Communication

Useful Links

Subgroup Strategies, Overview

Powerpoint presentations, drafts

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