IGEM:Berkeley/2010

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Berkeley iGEM 2010

Gel Pictures

Project Name

Project Summary

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Undergrads
Amy Kristofferson
Conor McClune
Tahoura
Daniela
Christoph

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson

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Lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Polymerase Reactions

Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange

Cleanup Reactions

Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions

EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations

Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Assays

Other

LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA


Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook

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Addressable Cell-to-Cell Communication

Useful Links


Subgroup Strategies, Overview

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